Fig. 7. Effect of mitochondria-bound HKII on tBHP-induced cell death in WT and VDAC1^-/- H9c2 cells. (A) Western blot analysis of HKII level in WT and VDAC1^-/- H9c2 cells from total cell lysate. β-tubulin level was used as protein loading control. (B) Western blot analysis of mitochondrial HKII levels in WT and VDAC1^-/- H9c2 cells. (C) Western blot analysis of mitochondrial HKII levels in WT and VDAC1^-/-C1 H9c2 cells exposed to tBHP. (D) Western blot analysis of HKII level in mitochondrial and cytosolic fractions in WT, VDAC1^-/- C1 and VDAC1^-/-C1+ restored VDAC1 H9c2 cells. *P<0.05 vs. WT. Each experiment was repeated three times.